Skip to main content
Addgene

pAFMW-Nbr-D435A/E437A
(Plasmid #40829)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40829 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAFMW
  • Backbone manufacturer
    Murhpy Lab, Carnegie Institution for Science
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Nbr D435A/E437A
  • Alt name
    Nibbler
  • Alt name
    Nbr
  • Species
    D. melanogaster (fly)
  • Mutation
    D435A/E437A, part of the DEDD motif; mutations predicted to block exonuclease activity
  • Entrez Gene
    Nbr (a.k.a. Dmel_CG9247, CG9247, Dmel\CG9247, nbr)
  • Promoter Actin5C
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on backbone)
    • 6xMyc (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAFMW-Nbr-D435A/E437A was a gift from Phillip Zamore (Addgene plasmid # 40829 ; http://n2t.net/addgene:40829 ; RRID:Addgene_40829)

  • For your References section:

    The 3'-to-5' exoribonuclease Nibbler shapes the 3' ends of microRNAs bound to Drosophila Argonaute1. Han BW, Hung JH, Weng Z, Zamore PD, Ameres SL. Curr Biol. 2011 Nov 22;21(22):1878-87. Epub 2011 Nov 3. 10.1016/j.cub.2011.09.034 PubMed 22055293
Related items:
Commonly requested with: