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Addgene

EGFP-alphasynuclein-WT
(Plasmid #40822)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40822 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SNCA
  • Alt name
    Alpha-synuclein
  • Species
    H. sapiens (human)
  • Entrez Gene
    SNCA (a.k.a. NACP, PARK1, PARK4, PD1)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that EcoRI is not a unique site. The alpha-synuclein insert contains an EcoRI site in the coding sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-alphasynuclein-WT was a gift from David Rubinsztein (Addgene plasmid # 40822 ; http://n2t.net/addgene:40822 ; RRID:Addgene_40822)

  • For your References section:

    Alpha-synuclein overexpression promotes aggregation of mutant huntingtin. Furlong RA, Narain Y, Rankin J, Wyttenbach A, Rubinsztein DC. Biochem J. 2000 Mar 15;346 Pt 3:577-81. 10.1042/0264-6021:3460577 PubMed 10698681
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