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Addgene

pMCL-HA-MAPKK1-11A55B [S218E/S222D]
(Plasmid #40809)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40809 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10200
  • Total vector size (bp) 11700
  • Modifications to backbone
    See attached map & info
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MAP2K1
  • Alt name
    MKK1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    S218E, S222D
  • GenBank ID
    NM_002755
  • Entrez Gene
    MAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCL-HA-MAPKK1-11A55B [S218E/S222D] was a gift from Natalie Ahn (Addgene plasmid # 40809 ; http://n2t.net/addgene:40809 ; RRID:Addgene_40809)

  • For your References section:

    Transformation of mammalian cells by constitutively active MAP kinase kinase. Mansour SJ, Matten WT, Hermann AS, Candia JM, Rong S, Fukasawa K, Vande Woude GF, Ahn NG. Science. 1994 Aug 12;265(5174):966-70. 10.1126/science.8052857 PubMed 8052857
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