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pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D]
(Plasmid #40806)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40806 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMM9 from pMB1
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 6900
  • Modifications to backbone
    pMB1: Blair et al. PNAS 77,3504 (1980)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418) ; G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MAP2K2
  • Alt name
    MKK2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    delta(48-55) + S222D + S226D
  • GenBank ID
    NM_030662
  • Entrez Gene
    MAP2K2 (a.k.a. CFC4, MAPKK2, MEK2, MKK2, PRKMK2)
  • Promoter MSV-LTR
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer MSV-LTR
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Kun-liang Guan provided gene, Dr. George Vande Woude provided vector backbone
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D] was a gift from Natalie Ahn (Addgene plasmid # 40806 ; http://n2t.net/addgene:40806 ; RRID:Addgene_40806)

  • For your References section:

    Megakaryocytic differentiation induced by constitutive activation of mitogen-activated protein kinase kinase. Whalen AM, Galasinski SC, Shapiro PS, Nahreini TS, Ahn NG. Mol Cell Biol. 1997 Apr;17(4):1947-58. PubMed 9121442
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