nuc4-
(Bacterial strain
#40803)
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Bacterial Strain | 40803 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
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Backbone manufacturerN/A
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Modifications to backboneN/A
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Vector typeN/A
- Promoter N/A
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)E. coli MG1655
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Copy numberUnknown
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
nuc4- was a gift from George Church (Addgene plasmid # 40803) -
For your References section:
Improving Lambda Red Genome Engineering in Escherichia coli via Rational Removal of Endogenous Nucleases. Mosberg JA, Gregg CJ, Lajoie MJ, Wang HH, Church GM. PLoS One. 2012;7(9):e44638. Epub 2012 Sep 5. 10.1371/journal.pone.0044638 PubMed 22957093