-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 40801 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneFUW-TetO
- Backbone size w/o insert (bp) 8339
- Total vector size (bp) 10539
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Stbl3
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCtcf
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)2200
-
Entrez GeneCtcf
- Promoter Minimal CMV + TetO
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CGC CTG GAG ACG CCA TCC ACG CTG
- 3′ sequencing primer AGA ATA CCA GTC AAT CTT TCA C (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
FUW-TetO-Ctcf was a gift from Rudolf Jaenisch (Addgene plasmid # 40801 ; http://n2t.net/addgene:40801 ; RRID:Addgene_40801) -
For your References section:
Single-Cell Expression Analyses during Cellular Reprogramming Reveal an Early Stochastic and a Late Hierarchic Phase. Buganim Y, Faddah DA, Cheng AW, Itskovich E, Markoulaki S, Ganz K, Klemm SL, van Oudenaarden A, Jaenisch R. Cell. 2012 Sep 14;150(6):1209-22. doi: 10.1016/j.cell.2012.08.023. 10.1016/j.cell.2012.08.023 PubMed 22980981
