Erk2
(Plasmid #40777)

• Purpose: Expression of rat Erk2 in mammalian cells
• Depositing Lab: Dustin Maly
• Publication: Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi: 10.1016/j.chembiol.2014.02.016. Epub 2014 Apr 3.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 7000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Erk2
• Species: Rattus norvegicus
• Insert Size (bp): 1000
• Mutation: Starting Met removed
• Entrez Gene: Mapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: N-GFP
• 3′ sequencing primer: BGHR

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry