Mek2
(Plasmid #40776)

• Depositing Lab: Dustin Maly
• Publication: various new plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3.1-Hygro
• Backbone size w/o insert (bp): 5600
• Total vector size (bp): 6800
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MKK2
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• Mutation: Starting Met removed
• Entrez Gene: MAP2K2 (a.k.a. CFC4, MAPKK2, MEK2, MKK2, PRKMK2)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry