DUSP6
(Plasmid
#40772)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 40772 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMCSG7
- Backbone size w/o insert (bp) 5200
- Total vector size (bp) 6300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameDUSP6
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SpeciesH. sapiens (human)
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Insert Size (bp)1100
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MutationStarting Met removed
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Entrez GeneDUSP6 (a.k.a. HH19, MKP3, PYST1)
- Promoter T7
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Tags
/ Fusion Proteins
- 6His (N terminal on insert)
- TEV (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCloned from addgene #27975
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DUSP6 was a gift from Dustin Maly (Addgene plasmid # 40772 ; http://n2t.net/addgene:40772 ; RRID:Addgene_40772) -
For your References section:
Conformation-Selective ATP-Competitive Inhibitors Control Regulatory Interactions and Noncatalytic Functions of Mitogen-Activated Protein Kinases. Hari SB, Merritt EA, Maly DJ. Chem Biol. 2014 May 22;21(5):628-35. doi: 10.1016/j.chembiol.2014.02.016. Epub 2014 Apr 3. 10.1016/j.chembiol.2014.02.016 PubMed 24704509