DUSP6
(Plasmid #40772)

• Depositing Lab: Dustin Maly
• Publication: Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi: 10.1016/j.chembiol.2014.02.016. Epub 2014 Apr 3.

Backbone

• Vector backbone: pMCSG7
• Backbone size w/o insert (bp): 5200
• Total vector size (bp): 6300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: DUSP6
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Mutation: Starting Met removed
• Entrez Gene: DUSP6 (a.k.a. HH19, MKP3, PYST1)
• Promoter: T7
• Tags / Fusion Proteins:
  • 6His (N terminal on insert)
  • TEV (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cloned from addgene #27975

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry