SETD7
(Plasmid #40746)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Toronto SGC Q1 Q2 2012 (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: SGC
• Modifications to backbone: C-term His tag added
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SETD7
• Alt name: SET domain containing (lysine methyltransferase) 7
• Alt name: PDB:4E47
• Species: H. sapiens (human)
• Insert Size (bp): 774
• GenBank ID: gi|83035135
• Entrez Gene: SETD7 (a.k.a. KMT7, SET7, SET7/9, SET9)
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

http://www.thesgc.org/structures/4E47/