DOT1L
(Plasmid
#40736)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 40736 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepET28-MHL
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Backbone manufacturerSGC
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameDOT1L
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Alt nameDOT1-like, histone H3 methyltransferase
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Alt nameDOT1; KMT4
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Alt namePDB: 4EQZ, 3UWP, 4ER0, 4ER3, 4ER5, 4ER6, 4ER7
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SpeciesH. sapiens (human)
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Insert Size (bp)1260
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GenBank IDgi|163965372
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Entrez GeneDOT1L (a.k.a. DOT1, KMT4)
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Tag
/ Fusion Protein
- 6xHis TEV (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DOT1L was a gift from Cheryl Arrowsmith (Addgene plasmid # 40736 ; http://n2t.net/addgene:40736 ; RRID:Addgene_40736)