pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b
(Plasmid #40731)

• Depositing Lab: Daniel Gerlich
• Publication: Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83. Epub 2012 Jul 16.

Backbone

• Vector backbone: pIRES-puro2b
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5125
• Total vector size (bp): 7062
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Aurora B FRET-sensor
• Alt name: CyPet-FHA2-Aurora B substrate-YPet
• Alt name: cytoplasmic Aurora B biosensor
• Species: Synthetic
• Insert Size (bp): 1937
• Promoter: CMV
• Tags / Fusion Proteins:
  • CyPet (N terminal on insert)
  • YPet (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: IRES-R

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: Michael Lampson, Department of Biology, University of Pennsylvania, Philadelphia, US

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry