pUB2.1
(Plasmid #40728)

• Purpose: (Empty Backbone) Modified pcDNA3.1 vector for ligation independent cloning, including a ccdB cassette for eliminating no-insert background
• Depositing Lab: Thomas Hughes
• Publication: pUB2.1 (unpublished)

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 5488
• Modifications to backbone: carries a negative selection cassette, ccdb, in the cloning site. Originally, pcDNA3.1 hygro, modified for ligation independent cloning with zero background.
• Vector type: Mammalian Expression
• Promoter: CMV

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • pUB2.1 protocol
• A portion of this plasmid was derived from a plasmid made by: We purchased the backbone from Invitrogen, and then adapted it for high-throughput cloning.
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the hygromycin cassette in this plasmid is non-functional. See provided protocol for high-throughput, ligation-independent cloning using this plasmid.