phage-ubc-nls-ha-tdMCP-gfp
(Plasmid #40649)

• Depositing Lab: Robert Singer
• Publication: Wu et al Biophys J. 2012 Jun 20;102(12):2936-44. Epub 2012 Jun 19.

Backbone

• Vector backbone: pHAGE-UBC-RIG (modified)
• Modifications to backbone: see associated publication
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tdMCP-gfp
• Alt name: tandem dimer MS2 coat protein (MCP)
• Species: Synthetic
• Promoter: human ubiquitin C (UBC)
• Tags / Fusion Proteins:
  • NLS (N terminal on backbone)
  • HA (N terminal on backbone)
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: FUGW (5'-ATTACAGGGACAGCAGAGATCC-3')
• 3′ sequencing primer: WPRE-R (5'CATAGCGTAAAAGGAGCAACA-3')

Resource Information

• Articles Citing this Plasmid:
  • 18 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The linker region between the two MCPs is ATCTACGCCATGGCTTCT