hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP
(Plasmid #40640)

• Depositing Lab: Brenda Lilly
• Publication: Zhao et al J Biol Chem. 2012 May 11;287(20):16111-20. Epub 2012 Mar 21.

Backbone

• Vector backbone: pCDF1-MCS2-EF1-copGFP
• Backbone manufacturer: System Biosciences
• Backbone size w/o insert (bp): 6771
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NICD3
• Alt name: NOTCH3 intracellular domain
• Species: H. sapiens (human)
• Insert Size (bp): 2086
• Entrez Gene: NOTCH3 (a.k.a. CADASIL, CADASIL1, CASIL, IMF2, LMNS)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI/NotI blunted (destroyed during cloning)
• 5′ sequencing primer: LNCX_primer
• 3′ sequencing primer: EF1a-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry