pGEX4T-1_OCT4_S235E
(Plasmid
#40638)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 40638 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX4T-1
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Backbone manufacturerGE-healthcare
- Backbone size w/o insert (bp) 6237
- Total vector size (bp) 7362
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Modifications to backboneAdded TEV cleavage site
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePOU5F1
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Alt nameOCT4
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SpeciesH. sapiens (human)
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Insert Size (bp)1125
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MutationS235E
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Entrez GenePOU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
- Promoter tac
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Tags
/ Fusion Proteins
- GST (N terminal on insert)
- Thrombin Cleavage (N terminal on insert)
- TEV Cleavage (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcorI (not destroyed)
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX4T-1_OCT4_S235E was a gift from James Thomson (Addgene plasmid # 40638 ; http://n2t.net/addgene:40638 ; RRID:Addgene_40638) -
For your References section:
Phosphorylation regulates human OCT4. Brumbaugh J, Hou Z, Russell JD, Howden SE, Yu P, Ledvina AR, Coon JJ, Thomson JA. Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 2. 10.1073/pnas.1203874109 PubMed 22474382
Map uploaded by the depositor.