pGEX4T-1_WT_OCT4
(Plasmid #40633)

• Depositing Lab: James Thomson
• Publication: Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 2.

Backbone

• Vector backbone: pGEX4T-1
• Backbone manufacturer: GE-healthcare
• Backbone size w/o insert (bp): 6237
• Total vector size (bp): 7362
• Modifications to backbone: Added TEV cleavage site
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: POU5F1
• Alt name: OCT4
• Species: H. sapiens (human)
• Insert Size (bp): 1125
• Entrez Gene: POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
• Promoter: tac
• Tags / Fusion Proteins:
  • GST (N terminal on insert)
  • Thrombin cleavage (N terminal on insert)
  • TEV cleavage (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry