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pCEP4_OCT4_T234A_S235A
(Plasmid #40630)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40630 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10712
  • Total vector size (bp) 11795
  • Vector type
    Mammalian Expression
  • Selectable markers
    NA

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    POU5F1_S234A_T235A
  • Alt name
    OCT4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1080
  • Mutation
    S234A_T235A
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
  • Promoter EF1-alpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4_OCT4_T234A_S235A was a gift from James Thomson (Addgene plasmid # 40630 ; http://n2t.net/addgene:40630 ; RRID:Addgene_40630)

  • For your References section:

    Phosphorylation regulates human OCT4. Brumbaugh J, Hou Z, Russell JD, Howden SE, Yu P, Ledvina AR, Coon JJ, Thomson JA. Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 2. 10.1073/pnas.1203874109 PubMed 22474382
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