p59sheLL
(Plasmid #40628)

• Depositing Lab: Simon Beddows
• Publication: Draper et al Vaccine. 2011 Nov 3;29(47):8585-90. Epub 2011 Sep 21.

Backbone

• Vector backbone: p5sheLL
• Backbone manufacturer: J.T. Schiller, National Cancer Institute, Bethesda, MD, USA (http://home.ccr.cancer.gov/lco/default.asp)
• Backbone size w/o insert (bp): 7862
• Total vector size (bp): 10784
• Modifications to backbone: HPV5 L1 and L2 replaced with HPV59 L1 and L2
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: HPV59 L1
• Alt name: L1 major capsid protein
• Alt name: L1
• Alt name: HPV 59L1
• Species: Human Papillomavirus Type 59
• Insert Size (bp): 1527
• Mutation: Codon optimized for mammalian expression
• GenBank ID: Please see comments below
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspEI (not destroyed)
• 3′ cloning site: PspXI (not destroyed)
• 5′ sequencing primer: CMV-F

Gene/Insert 2

• Gene/Insert name: HPV59 L2
• Alt name: L2 minor capsid protein
• Alt name: L2
• Alt name: HPV 59L2
• Species: Human Papillomavirus Type 59
• Insert Size (bp): 1395
• Mutation: Codon optimized for mammalian expression
• GenBank ID: Please see comments below
• Promoter: Polio IRES

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: Unknown
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • p59sheLL.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructs representing HPV35 (p35sheLL), HPV39 (p39sheLL) and HPV59 (p59sheLL) were generated by the insertion of codon optimized genes (Blue Heron, Inc., Bothell, WA, USA) based upon consensus L1 and L2 amino acid sequences into p5shell (http://home.ccr.cancer.gov/lco/default.asp) The consensus sequences were derived from NCBI database sequences (HPV35: M74117, X74477; HPV39: M62849; HPV59: X77858, EU918767) and contemporary sequences from anonymous, HPVinfected cytology samples (HPV35 L1: JN104062–64; HPV35 L2: JN104065–67; HPV39 L1: JN104068–70; HPV39 L2: JN104071–72; HPV59 L1: JN104073–74; HPV59 L2: JN104075–77).