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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 40625 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUGW-H1
- Backbone size w/o insert (bp) 10130
- Total vector size (bp) 10170
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Vector typeLentiviral, RNAi
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Selectable markersZeocin ; Zeo marker is outside the LTRs and will not be packaged into virus
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl2
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameScrambled control shRNA
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gRNA/shRNA sequenceTTCTCCGAACGTGTCACGT
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SpeciesM. musculus (mouse)
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GenBank ID
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This Scrambled control shRNA plasmid was generated by inserting the hairpin oligonucleotides into the FUGW-H1
lentiviral construct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FUGW-H1-Scrambled control shRNA was a gift from Sally Temple (Addgene plasmid # 40625 ; http://n2t.net/addgene:40625 ; RRID:Addgene_40625) -
For your References section:
The Niche Factor Syndecan-1 Regulates the Maintenance and Proliferation of Neural Progenitor Cells during Mammalian Cortical Development. Wang Q, Yang L, Alexander C, Temple S. PLoS One. 2012;7(8):e42883. Epub 2012 Aug 24. 10.1371/journal.pone.0042883 PubMed 22936997