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Addgene

pCDH-F-PA1
(Plasmid #40618)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40618 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-EF1-MCS-IRES-Neo
  • Backbone manufacturer
    System Biosciences (SBI)
  • Backbone size w/o insert (bp) 8295
  • Total vector size (bp) 9100
  • Vector type
    Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PA1
  • Alt name
    C16orf53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    750
  • GenBank ID
    NM_024516.3
  • Entrez Gene
    PAGR1 (a.k.a. C16orf53, GAS, PA1)
  • Promoter EF1
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba I (not destroyed)
  • 3′ cloning site BamH I (not destroyed)
  • 5′ sequencing primer 5'-ccctgcttgctcaactctac-3'
  • 3′ sequencing primer 5'-GCATTCCTTTGGCGAGAG-3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-F-PA1 was a gift from Kai Ge (Addgene plasmid # 40618 ; http://n2t.net/addgene:40618 ; RRID:Addgene_40618)

  • For your References section:

    UTX regulates mesoderm differentiation of embryonic stem cells independent of H3K27 demethylase activity. Wang C, Lee JE, Cho YW, Xiao Y, Jin Q, Liu C, Ge K. Proc Natl Acad Sci U S A. 2012 Sep 4. 10.1073/pnas.1204166109 PubMed 22949634
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