pCDH-UTY-F
(Plasmid #40617)

• Depositing Lab: Kai Ge
• Publication: Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Backbone

• Vector backbone: pCDH-EF1-MCS-IRES-Neo
• Backbone manufacturer: System Biosciences (SBI)
• Backbone size w/o insert (bp): 8295
• Total vector size (bp): 12400
• Vector type: Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UTY
• Alt name: ubiquitously transcribed tetratricopeptide repeat gene, Y-linked
• Species: H. sapiens (human)
• Insert Size (bp): 4000
• GenBank ID: NM_007125
• Entrez Gene: UTY (a.k.a. UTY1)
• Promoter: EF1
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba I (unknown if destroyed)
• 3′ cloning site: Xba I (unknown if destroyed)
• 5′ sequencing primer: 5'-ccctgcttgctcaactctac -3'
• 3′ sequencing primer: 5'-GCATTCCTTTGGCGAGAG-3'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry