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PurposeExpresses VP16-CIB1 for light-inducible protein expression when coupled with GalBD-CRY2PHR or GalBD-CRY2
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 40615 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGADT7rec
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 7500
- Total vector size (bp) 8776
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVP16-CIB1
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)1314
- Promoter ADH1
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRMH124-VP16-CIB1 was a gift from Chandra Tucker (Addgene plasmid # 40615 ; http://n2t.net/addgene:40615 ; RRID:Addgene_40615) -
For your References section:
Light-mediated control of DNA transcription in yeast. Hughes RM, Bolger S, Tapadia H, Tucker CL. Methods. 2012 Aug 15. 10.1016/j.ymeth.2012.08.004 PubMed 22922268
Map uploaded by the depositor.
