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Addgene

719-2µ-minHIS3
(Plasmid #40610)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40610 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBEVY-U
  • Backbone manufacturer
    Miller et al (1998) Nucleic Acids Res 26(15):3577-3583
  • Backbone size w/o insert (bp) 6512
  • Total vector size (bp) 7212
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIS3
  • Species
    S. cerevisiae (budding yeast), Synthetic
  • Insert Size (bp)
    700
  • Mutation
    Every codon of the coding sequence has been replaced with the isoaccepting codon decoded by the least abundant tRNA.
  • GenBank ID
    NM_001183621
  • Entrez Gene
    HIS3 (a.k.a. YOR202W, HIS10, HIS8)
  • Promoter TDH3
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer TDH3
  • 3′ sequencing primer M13r
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    719-2µ-minHIS3 was a gift from Tobias von der Haar (Addgene plasmid # 40610 ; http://n2t.net/addgene:40610 ; RRID:Addgene_40610)

  • For your References section:

    Translation elongation can control translation initiation on eukaryotic mRNAs. Chu D, Kazana E, Bellanger N, Singh T, Tuite MF, von der Haar T. EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.1002/embj.201385651. Epub 2013 Dec 19. 10.1002/embj.201385651 PubMed 24357599
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