AMPK-γ1-HA
(Plasmid #40605)

• Depositing Lab: Harvey Lodish
• Publication: Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-42. Epub 2006 Sep 29.

Backbone

• Vector backbone: pCMV-Tag4A
• Backbone size w/o insert (bp): 4300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AMPK-γ1
• Alt name: AMPK-gamma1
• Alt name: AMPK-G1
• Alt name: Prkag1
• Species: M. musculus (mouse)
• Entrez Gene: Prkag1 (a.k.a. AA571379, BB036179, Prkaac)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: CMV_fwd
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

AMPK-γ1 insert has Kozak sequence embedded at 5'-terminus and a stop codon at the 3'-terminus. The FLAG of the pCMV-Tag4a backbone is out of frame.