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Addgene

AMPK-β1-FLAG
(Plasmid #40602)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40602 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Tag4A
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AMPK-β1
  • Alt name
    AMPK-beta1
  • Alt name
    AMPK-B1
  • Alt name
    Prkab1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Prkab1 (a.k.a. 1300015D22Rik, AU021155, E430008F22)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PmeI/EcoRV (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV_fwd
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AMPK-β1-FLAG was a gift from Harvey Lodish (Addgene plasmid # 40602 ; http://n2t.net/addgene:40602 ; RRID:Addgene_40602)

  • For your References section:

    A revised model for AMP-activated protein kinase structure: The alpha-subunit binds to both the beta- and gamma-subunits although there is no direct binding between the beta- and gamma-subunits. Wong KA, Lodish HF. J Biol Chem. 2006 Nov 24;281(47):36434-42. Epub 2006 Sep 29. 10.1074/jbc.M607410200 PubMed 17012231
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