pTH646-2µ-RLuc
(Plasmid #40600)

• Depositing Lab: Tobias von der Haar
• Publication: Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.1002/embj.201385651. Epub 2013 Dec 19.

Backbone

• Vector backbone: pBEVY-U
• Backbone manufacturer: Miller et al (1998) Nucleic Acids Res 26(15):3577-3583
• Backbone size w/o insert (bp): 6518
• Total vector size (bp): 7466
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Renilla Luciferase
• Alt name: RLuc
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 948
• Promoter: ADH1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XmaI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: ADH1
• 3′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The RLuc gene originated from a plasmid kindly provided by Dr David Bedwell (University of Alabama, Birmingham). Salas-Marco J, Bedwell DM (2004) Molecular and Cellular Biology 24:7769-7778.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry