pTH760-CEN-mCherry_v3
(Plasmid #40598)

• Depositing Lab: Tobias von der Haar
• Publication: Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.1002/embj.201385651. Epub 2013 Dec 19.

Backbone

• Vector backbone: pRS316
• Backbone size w/o insert (bp): 4874
• Total vector size (bp): 6507
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
• Alt name: mCherry
• Species: Synthetic
• Insert Size (bp): 1633
• Mutation: mCherry ORF partially codon optimised for yeast expression
• Promoter: GPD (TDH3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: EagI (not destroyed)
• 5′ sequencing primer: M13f
• 3′ sequencing primer: M13r

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The cassette comprising promoter, mCherry ORF and transcription terminator was subcloned from a construct originally generated by Dr Wenfeng Qian and Prof Jianzhi Zhang, University of Michigan. Qian et al. (2012) Balanced codon usage optimizes eukaryotic translational efficiency. PLoS Genetics 8(3):e1002603.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry