pRMH120
(Plasmid #40593)

• Purpose: Expresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein expression
• Depositing Lab: Chandra Tucker
• Publication: Hughes et al Methods. 2012 Aug 15.

Backbone

• Vector backbone: p414TEF
• Backbone size w/o insert (bp): 5414
• Total vector size (bp): 10860
• Modifications to backbone: Gal4BD-CRY2PHR inserted at TEF MCS, pADH-Gal4AD-CIBFL-tADH inserted at KpnI site
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: CRY2
• Alt name: cryptochrome 2
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1500
• Entrez Gene: CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
• Promoter: TEF

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: CIB1
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1000
• Entrez Gene: CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
• Promoter: ADH1

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry