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Addgene

MIEG-hCD4
(Plasmid #40569)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40569 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMIEG3
  • Backbone manufacturer
    David A. Williams (PMID: 10961859)
  • Modifications to backbone
    IRES EGFP of pMIEG replaced with human CD4 cDNA
  • Vector type
    Mammalian Expression, Retroviral
  • Tag / Fusion Protein
    • IRES hCD4 (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer pLXSN_5
  • 3′ sequencing primer IRES-R2 (gacggcaatatggtggaaa)
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cloned human CD4 cDNA provided by G. Alkhatib, Indiana University.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Retroviral vector MIEG-hCD4 was made by replacing the EGFP of the MIEG-EGFP vector with the hCD4 cDNA amplified from a cloned cDNA (provided by G. Alkhatib, Indiana University).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MIEG-hCD4 was a gift from Alexander Dent & Mark Kaplan (Addgene plasmid # 40569 ; http://n2t.net/addgene:40569 ; RRID:Addgene_40569)

  • For your References section:

    PU.1 expression delineates heterogeneity in primary Th2 cells. Chang HC, Zhang S, Thieu VT, Slee RB, Bruns HA, Laribee RN, Klemsz MJ, Kaplan MH. Immunity. 2005 Jun;22(6):693-703. 10.1016/j.immuni.2005.03.016 PubMed 15963784
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