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Addgene

Gata3-MIEG-hCD4
(Plasmid #40568)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40568 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MIEG-hCD4
  • Backbone manufacturer
    Addgene plasmid 40569
  • Modifications to backbone
    IRES EGFP of pMIEG replaced with human CD4 cDNA
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gata3
  • Alt name
    Gata-3
  • Alt name
    GATA binding protein 3
  • Species
    M. musculus (mouse)
  • GenBank ID
    NM_008091.3 NP_032117.1
  • Entrez Gene
    Gata3 (a.k.a. Gata-3, jal)
  • Tag / Fusion Protein
    • IRES hCD4 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer pLXSN_5
  • 3′ sequencing primer IRES-R2 (gacggcaatatggtggaaa)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The coding region for murine GATA-3 cDNA was amplified by PCR and cloned into MIEG-hCD4. Please note that the discrepancies between the QC and NCBI sequences should not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gata3-MIEG-hCD4 was a gift from Alexander Dent & Mark Kaplan (Addgene plasmid # 40568 ; http://n2t.net/addgene:40568 ; RRID:Addgene_40568)

  • For your References section:

    PU.1 expression delineates heterogeneity in primary Th2 cells. Chang HC, Zhang S, Thieu VT, Slee RB, Bruns HA, Laribee RN, Klemsz MJ, Kaplan MH. Immunity. 2005 Jun;22(6):693-703. 10.1016/j.immuni.2005.03.016 PubMed 15963784
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