pEMB52/Armadillo_34_373
(Plasmid
#40543)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 40543 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepEMB52
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Backbone manufacturerEmerald
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Vector typeBaculovirus
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLRRK2
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SpeciesDasypus novemcinctus
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MutationWT
- Promoter polH
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site HindIII (destroyed during cloning)
- 5′ sequencing primer DSP2466For:GAATTTCGTCGACAGATC
- 3′ sequencing primer DSP2466Rev:AATAACCCGGATCAGCTT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEMB52/Armadillo_34_373 was a gift from Michael J Fox Foundation MJFF (Addgene plasmid # 40543 ; http://n2t.net/addgene:40543 ; RRID:Addgene_40543)