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Addgene

pBACgus_Sumostar/LRRK2 1867-2161_C2024S/C2025S
(Plasmid #40394)

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Item Catalog # Description Quantity Price (USD)
Plasmid 40394 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pBACgus_SUMOstar
  • Backbone manufacturer
    Novagen
  • Vector type
    Baculovirus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SUMO
  • Species
    Synthetic
  • Promoter polH
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer DSP2466For:GAATTTCGTCGACAGATC
  • 3′ sequencing primer DSP2466Rev:AATAACCCGGATCAGCTT
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    LRRK2
  • Species
    H. sapiens (human)
  • Mutation
    aa1867-2161,C2024S/C2025S
  • Entrez Gene
    LRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
  • Tag / Fusion Protein
    • HisTEV (N terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBACgus_Sumostar/LRRK2 1867-2161_C2024S/C2025S was a gift from Michael J Fox Foundation MJFF (Addgene plasmid # 40394 ; http://n2t.net/addgene:40394 ; RRID:Addgene_40394)
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