pMIEG3-c-Jun
(Plasmid #40348)

• Depositing Lab: Alexander Dent
• Publication: Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Backbone

• Vector backbone: pMIEG3
• Backbone manufacturer: David A. Williams (PMID: 10961859)
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: c-Jun
• Alt name: jun proto-oncogene
• Species: H. sapiens (human)
• GenBank ID: NM_002228.3 NP_002219.1
• Entrez Gene: JUN (a.k.a. AP-1, AP1, c-Jun, cJUN, p39)
• Tag / Fusion Protein:
  • IRES eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: pLXSN_5
• 3′ sequencing primer: IRES-R2 (gacggcaatatggtggaaa)

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The c-Jun- and JunB-expressing retroviruses were constructed by inserting c-Jun and JunB cDNAs into the retroviral vector pMIEG3 via NotI and XhoI sites.