pMIEG3-BCL6
(Plasmid #40339)

• Depositing Lab: Alexander Dent
• Publication: Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Backbone

• Vector backbone: pMIEG3
• Backbone manufacturer: David A. Williams (PMID: 10961859)
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BCL6
• Alt name: BCL-6
• Alt name: B-cell CLL/lymphoma 6
• Species: H. sapiens (human)
• Mutation: optimized translation initiation codon
• GenBank ID: NG_007149.1
• Entrez Gene: BCL6 (a.k.a. BCL5, BCL6A, LAZ3, ZBTB27, ZNF51)
• Tag / Fusion Protein:
  • IRES eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: pLXSN_5
• 3′ sequencing primer: IRES-R2 (gacggcaatatggtggaaa)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Human BCL-6 cDNA with optimized translation initiation codon obtained from L.M. Staudt, NCI, NIH.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pMIEG3 plasmid was obtained from D.A. Williams (Indiana University School of Medicine). pMIEG3 is a murine stem cell virus (MSCV) retrovirus construct in which the gene encoding GFP is driven by the MSCV long terminal repeat. pMIEG3 contains an IRES that allows coexpression of the GFP gene and a second gene. The human BCL-6 cDNA with an optimized translation initiation codon (obtained from L.M. Staudt, NCI, NIH) was cloned into pMIEG3 via NotI and XhoI restriction sites.