ElectricPk
(Plasmid
#40314)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 40314 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUB2.1
- Backbone size w/o insert (bp) 5492
- Total vector size (bp) 6964
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameElectricPk
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Insert Size (bp)1472
- Promoter CMV
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Tag
/ Fusion Protein
- cpEGFP (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer bGH Poly A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please see the associated publication for more information: Barnett L, Platisa J, Popovic M, Pieribone VA, Hughes T (2012) A Fluorescent, Genetically-Encoded Voltage Probe Capable of Resolving Action Potentials. PLoS ONE 7(9): e43454. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0043454
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ElectricPk was a gift from Thomas Hughes (Addgene plasmid # 40314 ; http://n2t.net/addgene:40314 ; RRID:Addgene_40314) -
For your References section:
A fluorescent, genetically-encoded voltage probe capable of resolving action potentials. Barnett L, Platisa J, Popovic M, Pieribone VA, Hughes T. PLoS One. 2012;7(9):e43454. Epub 2012 Sep 6. 10.1371/journal.pone.0043454 PubMed 22970127