pCAG APX W41F dimeric (mito matrix)
(Plasmid #40307)

• Depositing Lab: Alice Ting
• Publication: Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375.

Backbone

• Vector backbone: pCAG
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5651

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pea APX W41F (dimeric)
• Alt name: CAG promoter, expression in mitochondrial matrix
• Species: Synthetic
• Insert Size (bp): 747
• Mutation: W41F mutation.
• Promoter: CAG
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCAG-F

Resource Information

• Supplemental Documents:
  • APEX2 Recommendations from Ting Lab
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is for expression of pea APX W41F (dimeric) in the mitochondrial matrix under the CAG promoter. In this construct, APX has a mitochondrial localization peptide (MLATRVFSLVGKRAISTSVCVRAH) fused to its N-terminus. The APX gene is mammalian codon optimized.