pUC57 APEX
(Plasmid #40306)

• Purpose: A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).
• Depositing Lab: Alice Ting
• Publication: Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375.

Backbone

• Vector backbone: pUC57
• Backbone manufacturer: Genscript
• Backbone size w/o insert (bp): 2710
• Total vector size (bp): 3457
• Vector type: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pea APEX (Synthetic)
• Alt name: pea APX with K14D, W41F, E112K mutations
• Species: Synthetic
• Insert Size (bp): 747
• Mutation: K14D, W41F, E112K (relative to wild-type)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pUC57 Forward (GTAAAACGACGGCCAGTG)
• 3′ sequencing primer: pUC57 Reverse (GGAAACAGCTATGACCATG)

Resource Information

• Supplemental Documents:
  • APEX2 Recommendations from Ting Lab
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This gene encodes APEX, a monomeric and improved-activity variant of pea ascorbate peroxidase. This plasmid is not for mammalian expression and should instead be used for PCR amplification of the APEX insert. The gene is mammalian codon optimized.