pFRT/FLAG/HA-DEST PUM2
(Plasmid #40292)

• Depositing Lab: Thomas Tuschl
• Publication: Hafner et al Cell. 2010 Apr 2;141(1):129-41.

Backbone

• Vector backbone: pFRT/FLAG/HA-DEST
• Backbone size w/o insert (bp): 5372
• Total vector size (bp): 8567
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pumilio homolog 2
• Alt name: PUM2
• Species: H. sapiens (human)
• Insert Size (bp): 3195
• GenBank ID: NM_015317
• Entrez Gene: PUM2 (a.k.a. PUMH2, PUML2)
• Tag / Fusion Protein:
  • FLAG-HA (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV
• 3′ sequencing primer: BGHR

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that there are some cloning scars flanking the insert (see Addgene sequence), but they will not affect plasmid function.