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Addgene

perbB2K732M-ECFP
(Plasmid #40269)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40269 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    erbB2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3765
  • Mutation
    K732M
  • Entrez Gene
    ERBB2 (a.k.a. CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, NEU, NGL, TKR1, VSCN2, c-ERB-2, c-ERB2, p185(erbB2))
  • Promoter CMV
  • Tag / Fusion Protein
    • ecfp (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site nheI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequence shows that the mutation is actually K753M. The discrepancy in the numbering is due to the presence of a cleaved signal peptide making the mature protein shorter.

There is also an I655V polymorphism in the sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    perbB2K732M-ECFP was a gift from Martin Offterdinger (Addgene plasmid # 40269 ; http://n2t.net/addgene:40269 ; RRID:Addgene_40269)
  • For your References section:

    Prolonged EGFR signaling by ERBB2-mediated sequestration at the plasma membrane. Offterdinger M, Bastiaens PI. Traffic. 2008 Jan;9(1):147-55. Epub 2007 Nov 13. 10.1111/j.1600-0854.2007.00665.x PubMed 17956594