perbB2K732M-ECFP
(Plasmid #40269)

• Depositing Lab: Martin Offterdinger
• Publication: Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007 Nov 13.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: erbB2
• Species: H. sapiens (human)
• Insert Size (bp): 3765
• Mutation: K732M
• Entrez Gene: ERBB2 (a.k.a. CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, NEU, NGL, TKR1, VSCN2, c-ERB-2, c-ERB2, p185(erbB2))
• Promoter: CMV
• Tag / Fusion Protein:
  • ecfp (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: nheI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: CMV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequence shows that the mutation is actually K753M. The discrepancy in the numbering is due to the presence of a cleaved signal peptide making the mature protein shorter.

There is also an I655V polymorphism in the sequence.