pHM6-Q74
(Plasmid #40264)

• Depositing Lab: David Rubinsztein
• Publication: Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Backbone

• Vector backbone: pHM6
• Backbone manufacturer: Roche
• Backbone size w/o insert (bp): 5450
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HTT partial exon 1 Q74
• Alt name: huntingtin
• Species: H. sapiens (human)
• Mutation: expanded poly-Q
• Entrez Gene: HTT (a.k.a. HD, IT15, LOMARS)
• Promoter: CMV
• Tags / Fusion Proteins:
  • HA (N terminal on backbone)
  • 6x His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry