pcDNA3-AKAR2-CR
(Plasmid #40255)

• Depositing Lab: Michael Lin
• Publication: Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5489
• Total vector size (bp): 7398
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AKAR2
• Alt name: forkhead associated domain 1 (FHA1) (Rad53p 22–162)
• Species: Synthetic
• Insert Size (bp): 1909
• Promoter: CMV IE
• Tags / Fusion Proteins:
  • His6 (N terminal on backbone)
  • Xpress (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The AKAR2 plasmid was gifted by J. Zhang (Department of Pharmacology and Molecular Sciences, John Hopkins Medicine).
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ECFP was replaced with GFP Clover and Citrine was replaced with RFP mRuby2.

Note: there is a small sequence insertion downstream of the insert that adds restriction sites. This is not present in the full plasmids sequence, so please refer to Addgene's QC sequence with the BGH-Rev primer for additional information.