pAM3558
(Plasmid #40252)

• Depositing Lab: Susan Golden
• Publication: Chen et al Plasmid. 2008 May;59(3):176-92. Epub 2008 Mar 18.

Backbone

• Vector backbone: pAM1303
• Total vector size (bp): 5300
• Vector type: Cyanobacteria cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Gm cassette (BssHII+SphI)
• Species: Synechococcus elongatus PCC 7942
• Insert Size (bp): 1200
• Promoter: n/a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • GenBank File
  • Strain Database Information

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NS1 vector

Gm resistant version of pAM1303.

Both pAM1303 and pAM3511 were digested with BssHII and SphI, and then mix-ligated. The accC1gene (Gm) replaces the aadA gene (SpSm) in the Ω cassette. The upstream T4 terminator was lost due to SphI digestion.