pAM1619
(Plasmid #40244)

• Depositing Lab: Susan Golden
• Publication: Mackey et al Methods Mol Biol. 2007;362:115-29.

Backbone

• Vector backbone: pAM1607
• Total vector size (bp): 410
• Vector type: Cyanobacteria cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PpsbAI::luxCDE
• Species: Synechococcus elongatus PCC 7942
• Insert Size (bp): 410
• Promoter: n/a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • GenBank File
  • Strain Database Information

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NSII vector

Lux Genes: luxCDE

Directs luciferase substrate synthesis in PCC7942. Synechococcus luxAB reporter strains glow without decanal addition

PpsbAI fragment as SalI-PvuII fragment from pAM1335 inserted upstream of luxCDE in SalI-StuI of pAM1607 (NSII, Km, luxCDE).