pAM1303
(Plasmid #40243)

• Depositing Lab: Susan Golden
• Publication: Kulkarni et al Mol Microbiol. 1997 Jun;24(6):1131-42.

Backbone

• Vector backbone: pAM1290
• Total vector size (bp): 5700
• Vector type: Cyanobacteria cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Sp/Sm Resistance, neutral site I integration platform for Synechococcus
• Species: Synechococcus elongatus PCC 7942
• Insert Size (bp): 14
• Promoter: n/a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • GenBank File
  • Strain Database Information
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NS1 vector

Cloning sites: NotI, BamHI and SmaI.

insert: GATCGGCGGCCGCG .........
...........CCGCCGGCGCCTAG NotI can be used as a primer site. Transforms and conjugates as well as pAM1175. Put in BamHI site of pAM1290 ; keeps a unique BamHI site on right.