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Addgene

pAM1706
(Plasmid #40242)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40242 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAM1667
  • Vector type
    Cyanobacteria cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PpsbAI
  • Species
    Synechococcus elongatus PCC 7942
  • Insert Size (bp)
    500
  • Promoter n/a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NS2.1 vector

Lux Genes: luxCDE

Directs luciferase substrate synthesis in PCC7942

500bp PvuII-SalI promoter fragment of psbAI inserted into SalI-StuI digested pAM1667 (luxCDE).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM1706 was a gift from Susan Golden (Addgene plasmid # 40242 ; http://n2t.net/addgene:40242 ; RRID:Addgene_40242)

  • For your References section:

    Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Andersson CR, Tsinoremas NF, Shelton J, Lebedeva NV, Yarrow J, Min H, Golden SS. Methods Enzymol. 2000;305:527-42. PubMed 10812624
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