pAM1579
(Plasmid #40240)

• Depositing Lab: Susan Golden
• Publication: Andersson et al Methods Enzymol. 2000;305:527-42.

Backbone

• Vector backbone: 1572
• Total vector size (bp): 8082
• Vector type: Cyanobacteria cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Km Resistance, neutral site II integration platform for Synechococcus
• Species: Synechococcus elongatus PCC 7942
• Insert Size (bp): 1500
• Promoter: n/a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • GenBank File
  • Strain Database Information
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NSII vector

cloning sites: NheI, XbaI, StuI, SalI, and EcoRV.

Km cassette into Klenow blunted BglII site of pAM1572. XhoI digest gives 0.7kb band. NSII vector; unique cloning sites NheI, XbaI, StuI, SalI, EcoRV.