pAM1518
(Plasmid #40238)

• Depositing Lab: Susan Golden
• Publication: Andersson et al Methods Enzymol. 2000;305:527-42.

Backbone

• Vector backbone: pAM1504
• Total vector size (bp): 12000
• Vector type: Cyanobacteria cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: psbAI promoter, luxCDE
• Species: Synechococcus elongatus PCC 7942
• Insert Size (bp): 1400
• Promoter: n/a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • Strain Database Information

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NS1 vector

Lux Genes: luxCDE

Directs luciferase substrate synthesis in PCC7942.

psbAI promoter fragment from pAM1469 (NotI-BglII fragment) was cloned in pAM1504 cut with NotI-BamHI, upstream luxCDE. This plasmid directs aldehyde biosynthesis in cyanobacteria.