pRS413-GAL1-luc*(-SKL)
(Plasmid #40234)

• Depositing Lab: Michael Benton
• Publication: pFA6-yEGFP1-HIS3MX6 (unpublished)

Backbone

• Vector backbone: pRS413
• Backbone size w/o insert (bp): 1650
• Total vector size (bp): 7314
• Vector type: Bacterial Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: luc*(-SKL)
• Alt name: Firefly luciferase
• Alt name: Photinus pyralis luciferase
• Alt name: luciferase, Fluc
• Species: Photinus pyralis
• Insert Size (bp): 1650
• Promoter: GAL1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: SmaI (not destroyed)
• 5′ sequencing primer: CATCGACTGAAATCCCTGGT
• 3′ sequencing primer: ccctccgaaggaagactctc

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mumberg D, Muller R and Funk M. 1995. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156(1): 119-122. Leskinen P, Virta M and Karp M (2003). One-step measurement of firefly luciferase activity in yeast. Yeast 20(13): 1109-1113. Bonin AL, Gossen M and Bujard H (1994). Photinus pyralis luciferase: vectors that contain a modified luc coding sequence allowing convenient transfer into other systems. Gene 141(1): 75-77.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry