SCORE
(Plasmid #40232)

• Depositing Lab: Wolfhard Almers
• Publication: An et al Science. 2004 Nov 5;306(5698):1042-6.

Backbone

• Vector backbone: peYFP-N1
• Backbone manufacturer: clontech
• Backbone size w/o insert (bp): 4733
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Snap25b
• Alt name: score
• Species: H. sapiens (human)
• Insert Size (bp): 2164
• GenBank ID: NM_130811
• Entrez Gene: SNAP25 (a.k.a. CMS18, RIC-4, RIC4, SEC9, SNAP, SNAP-25, SUP, bA416N4.2, dJ1068F16.2)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Venus (C terminal on insert)
  • CFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI, BspEI, AgeI, XhoI (unknown if destroyed)
• 3′ cloning site: BcII (unknown if destroyed)
• 5′ sequencing primer: CMV sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene found E713 in the SCORE sensor was Alanine when compared to the depositor's sequence. This result matches the expected Snap25 sequence (GenBank IDNM_130811).